To further confirm the proinflammatory role of WISP1 in the pathogenesis of IBD and whether administration of rWISP1 could aggravate TNBS-induced colitis, we administered rWISP1 into BALB/c mice during TNBS-induced colitis and found that rWISP1 exacerbated intestinal inflammation, characterized by more weight loss, shorter colon length, and severe clinical features as well as higher pathological scores. This evidence concerns the gene CCN4 and inflammatory bowel disease.