For accurate interpretation of the expression profiles of target genes, we concluded that EF, EIF4A and GAPDH were suitable for use as normalizers for different developmental stages and body parts of larval ghost moths, whereas EF, EIF4A and RPL13A were suitable for the low-temperature challenge and EF, EIF4A and TUB were suitable for the fungal infection and dietary treatments. Here, TUB is linked to fungal infectious disease.