Quantitative analysis demonstrated that the number of apoptotic cells (TUNEL-positive cells) was highest in HepG2 cells treated with 5-FC/GCV and ultrasound plus CMBsαvβ3 (p<0.05).Therefore this CMBsαvβ3-based CD/TK suicide gene delivery system is definitely effective and an important advance that could be translated clinically to improve suicide gene therapy for liver cancer and other malignant tumors, though in vivo experimental research are warranted before its clinical translation. This evidence concerns the gene TKT and liver cancer.