To compare the biological response to inhibition of Pol I transcription initiation of cancer cells to those of primary cell, we utilized the tumorigenic LSTR cell line, an isogenic BJ-derived cell line expressing SV40 early region (large-T and small-t), TERT, and an oncogenic allele of the HRAS gene (HRASG12V), which can grow tumours in nude mice [44]. The gene discussed is TERT; the disease is cancer.