The biological impact of PATZ1and PP4R2/PP4C negative feedback on IKK/NF-κB was further elucidated by upregulating and downregulating PATZ1 and PP4R2 to study their influence on the cellular effects of growth factors, PGE2 and PIP3 and the migration/invasion and colonization/metastasis of lung cancer cells in vitro and in vivo. The gene discussed is NFKB1; the disease is lung cancer.