Clinically, NOTCH1 transcript expression significantly correlate with non-smoking habit of patients, consistent with previous reports in other pathological conditions including lung adenocarcinoma [31-33]; lymph node metastasis in tongue cancer correlate with poor prognosis and survival of the patients, thus activated NOTCH1 could serve as a reliable marker to predict lymph node metastasis [35]. This evidence concerns the gene NOTCH1 and metastatic malignant neoplasm in the lymph nodes.