To investigate whether Maspin, RhoGDIα and CAPZB exert the similar function in HBxΔ31-mediated HCC metastasis, we over-expressed HBxΔ31 with or without Maspin, RhoGDIα or CAPZB in HepG2 cells, and examined their metastasis both in vitro and in vivo. The gene discussed is CAPZB; the disease is hepatocellular carcinoma.