Low-level tumor LINE-1 methylation was associated with higher pN stage and metastatic disease, and inversely associated with poor tumor differentiation, MSI-high, MLH1 hypermethylation, CIMP-high, and BRAF mutation (P ≤ 0.003 with the adjusted α level of 0.003 for multiple hypothesis testing). This evidence concerns the gene BRAF and metastatic neoplasm.