Herein, we explored a possible contribution of FGF-23 and Klotho to the genesis of vascular stiffening, endothelial dysfunction and oxidative stress by testing whether DPP-4 inhibition prevents the increase in aortic FGF-23 expression and modulates Klotho expression in the aorta of WD-fed mouse model. The gene discussed is DPP4; the disease is endothelial dysfunction.