Clinical characteristics of all 126 HCC patients (84 male and 42 female) were shown in Table 1: 57.1 % had HBV-related hepatitis; 24.6 % had HCV-related hepatitis; 37.3 % had small tumors (single nodule and diameter ≤2 cm); and 30.1 % had AFP-negative HCC (<20.0 ng/mL). The gene discussed is AFP; the disease is hepatocellular carcinoma.