To determine if genes related to metabolism of sugars released from glycan are involved during infection, nanT (STM3338; sialic acid transporter), xylR (STM3662; xylose operon regulatory protein), and bax (STM3663; hypothetical protein similar to ATP-binding protein) were deleted using homologous recombination20, characterized in their capacity to infect, and for alteration in invasion in vitro with Caco-2 cells (Fig. 1B). The gene discussed is BAX; the disease is infection.