PFKFB3 and overnutrition: This was expected as the supra-physiological glucose concentration used (27.5 mmol/L) models overnutrition and was necessary for this study to best demonstrate glucose regulation of PFKFB3. However, the control of inflammatory responses induced by such conditions likely requires more than one anti-inflammatory mechanism and thus, the anti-inflammatory ability of PFKFB3/iPFK2 alone may not be sufficient to fully suppress an inflammatory response of this nature.