In particular, p4EBP1 was rapidly inhibited following AR-A014418 treatment at 30 min in ACHN cells (Fig. 3a) and within 1 h in acquired rapamycin-resistant ACHN cells (Fig. 6c), supporting the direct involvement of GSK-3β in 4EBP1 phosphorylation in RCC cells. This evidence concerns the gene EIF4EBP1 and renal cell carcinoma.