To validate our in vitro observations and investigate whether FNDC3B could serve as therapeutic target for metastatic HCC, we established a stable cell line of Mahlavu cells with knockdown of FNDC3B. The stable knockdown cells or the control Mahlavu cells were mixed with matrigel and injected into nude mice by orthotopic intrahepatic or tail vein injection. Here, FNDC3B is linked to hepatocellular carcinoma.