Western blot analysis of the ovarian cancer cells shows an increase in apoptotic markers, such as cleaved PARP, cleaved caspase-3, Apaf-1, Bim, Bad, and Bid, in the si-Cdk1 transfected cells than in the si-negative control cells, in a time-dependent manner (Figure 3D). This evidence concerns the gene CDK1 and ovarian cancer.