Given that glucose uptake is mainly mediated by glucose transporter 1 (Glut1) in cancer cells, and that Glut1 expression is controlled by hypoxia inducible factor 1a (HIF-1α) [25], we examined expression of Glut1 and HIF-1α target genes in V-ATPase V1E1 depleted cells. The gene discussed is HIF1A; the disease is cancer.