Incubation of 7 days, as in the 3D culture assays, was performed on two malignant cancer cell lines: HT1080 fibrosarcoma cell line, and WM-115 human melanoma cell line (Figure 5B and 5C) after knock-down (Figure 5A) of endogenous rpS3 by si-RNAs (human si-rpS3/777 and si-rpS3/796). The gene discussed is RPS3; the disease is melanoma.