To confirm that TRPC6 channel-dependent [Ca2+]i elevation and successive activation of the extrinsic apoptotic signaling pathway induce neonatal GMC apoptosis, we measured HF-induced caspase-3/7 activation in neonatal pig GMCs pretreated with DMSO (control), BAPTA, and inhibitors of NFAT, Fas, and caspase-3/7. Here, CASP3 is linked to hydrops fetalis.