The shorter period of stable t-PA inhibition by NSP appears to be biologically meaningful however, as NSP interaction with t-PA in vivo has been identified as a significant negative-regulator of t-PA mediated effects on ischemic stroke (Yepes et al., 2000), seizure propagation (Yepes et al., 2002) and seizure induced blood brain barrier dysregulation (Fredriksson et al., 2015). Here, PLAT is linked to ischemic stroke.