SLC12A3 and Hypertension: 2015). This has also suggested that a vascular component could be contributing to the hypertension in addition to the salt retention caused by NCC activation in the DCT. However, the basis for the hyperkalemia was not explored and this forms the basis for this study. Since ROMK is the principal K+ secreting channel in the kidney, we have studied the effect of the CUL3 mutation on the WNK4 regulation of this channel using both in vitro and in vivo approaches.