MBD2 and breast carcinoma: Methylated DNA fragments were selected using a recombinant protein containing the Methyl‐CpG‐binding domain of MBD2 of MBD2 (Methyl‐Cap‐seq), from MDA‐MB‐231 cell line derived from human breast cancer, and the HMLER cell line constructed from in vitro transformation of human mammary cells (Elenbaas et al, 2001; Morel et al, 2008).