The in vitro cytotoxic assay showed potent effect of TF-8arm-PEG-DHA NPs against LLC and A549 cells, there is only 19.2% LLC and 33.2% A549 cells survival after 72 h; however, it exists a little difference due to the relative difference in cell surface TFR expression levels and suggests a highly selective nature for tumor cells that overexpress TFRs. Here, TFRC is linked to neoplasm.