EDN1 and liver dysplastic nodule: In addition, ERK has been shown to phosphorylate p66Shc at Ser36 in tubular cells41, and ET-1 can also induce MEK/ERK-dependent p66Shc serine phosphorylation42, indicating that many of these above events might participate in phosphorylation of p66Shc, resulting in tubular oxidative injury in patients with DN.