To assess whether Nod2 regulates the clearance parasite in vivo, WT and Nod2−/− mice were infected with sub-lethal doses of tachyzoites (Fig. 3C), and the parasite burden was evaluated after 5 days of infection in peritoneal exudate cells (Fig. 3D) and pancreas (Fig. 3E). This evidence concerns the gene NOD2 and infection.