Comparable results were observed by clonogenic assay when the siRNA-mediated depletion of BRCA1 and/or PARG was performed in the BRCA1-proficient U2OS osteosarcoma cell line, with even a slight increase in clonogenic survival of cells simultaneously depleted for PARG and BRCA1 compared to cells depleted for BRCA1 only (Fig. 3b). The gene discussed is BRCA1; the disease is osteosarcoma.