To determine whether inhibition of Pim-1 kinase by the pan-Pim kinase inhibitor AZD1208 enhances the growth inhibitory and pro-apoptotic effects of chemotherapy drugs in FLT3-ITD AML cells, the IC50 concentrations of DNR, MXR, VP-16 and AraC in FLT3-ITD- and FLT3-WT-expressing cell lines were first measured using the WST colorimetric assay (Supplementary Figure S1; Supplementary Table S1). Here, FLT3 is linked to acute myeloid leukemia.