We used Ba/F3 as our main cell system to evaluate the differential effect of inhibiting Pim kinase in cells expressing FLT3-ITD (Ba/F3-ITD) vs FLT3-WT (Ba/F3-WT), and subsequently studied another paired set of cell lines, 32D-ITD and 32D-WT, human cell lines and, importantly, primary AML cells. Here, FLT3 is linked to acute myeloid leukemia.