Flow cytometry analysis of renal cell suspensions showed that when compared with WT, C5aR1-/- kidneys had lower numbers of leukocytes (CD45+) at day 2 after infection and a lower proportion of MO/MΦs (Ly6G-CD11b+) within CD45+ cells at day 14 after infection (Figure 3a–c). This evidence concerns the gene C5AR1 and infection.