PTCH1 and breast cancer: Thus, we first evaluated by quantitative real time PCR (qRT-PCR) the expression of different members of Hedgehog signaling pathway, Shh, Ihh and Dhh ligands, their specific receptors, Patched 1 and 2 (Ptch1 and Ptch2) and some related genes such as Zeb1, Zeb2, ALCAM and VEGFA in BC cells treated with CPS for 120 h.