EIF2AK3 and familial pancreatic carcinoma: To further delineate, if NO actually induces the phosphorylation of FOXO3, we treated the human pancreatic cancer cell lines, Capan2 and SU.86.86 with a nitric oxide donor, Spermine/NONOate (Sper/NO), which resulted in an increased expression of both pERK and pFOXO3 (Figure 6B).