In addition, our in vivo results were further confirmed by reconstituting Gbp4-Asc complexes in HEK293T cells where both WT and GTPase-deficient mutant were found to form a macromolecular complex with Asc while CARD-deficient Gbp4 was unable to interact with the Asc speck and hardly rescued the high infection susceptibility of Gbp4-deficient larvae. The gene discussed is PYCARD; the disease is infection.