To determine whether DNAH8 affects androgen-dependent and -independent proliferation of prostate cancer cells, we depleted DNAH8 by siRNA in C4-2 (full-length AR) and 22Rv1 (full-length AR and AR splicing variant) cells, in both the absence and presence of androgens (Figure 5E).While these cell lines can proliferate in the absence of androgens (Figure 5C), they are AR-dependent as AR depletion suppressed cell proliferation (Supplementary Figure S3). This evidence concerns the gene AR and Familial prostate cancer.