Multiple regression analysis (R2 = 0.345; p = 0.032) showed that methylation at FOXP3 enhancer, sequence 2, CpG 5 was weakly influenced by smoking (p = 0.067) and older age (p = 0.073) but not by depression (p = 0.267) or antidepressants (p = 0.868). The gene discussed is FOXP3; the disease is major depressive disorder.