Functional experiments using Hep3B and Huh7 HCC cell lines and HepG2 hepatoblastoma cell line (Figure 8) showed that forced YAP1 overexpression was associated with a significant increase in the viability and mRNA levels of the stem cell markers CD133, NANOG, and OCT-3/4 in the three cell lines. This evidence concerns the gene PROM1 and hepatoblastoma.