The A549/pr(IFNβ).GFP reporter cell line provides a straightforward method to detect activation of the IFN induction pathway via eGFP expression under the control of the IFNβ promoter following stimulation by virus infection.12,13 We previously used this A549/pr(IFNβ).GFP reporter assay to demonstrate that existing IFN inhibitors block the IFN induction pathway.12 We propose here that the A549/pr(IFNβ).GFP reporter assay can be used to identify novel compounds that modulate the IFN induction pathway. This evidence concerns the gene IFNA1 and viral infectious disease.