MYC and colorectal cancer: Given SQLE’s MYC-independent prognostic ability (Fig. 4e, g), association with hypoxia in breast and colorectal cancer samples (Spearman’s ρ: Breast-Metabric = 0.31, Breast-BRCA = 0.42, P < 10−3; COADREAD = 0.17, P = 0.003) and hypoxic induction in three cell lines amongst those tested (Fig. 5c), we focussed on functional analysis of SQLE. Breast and colorectal cancer cells were treated with SQLE inhibitor (NB-598) under hypoxia and normoxia (Fig. 5g–j).