Given the positive correlation of five candidate metabolic genes located at MYC amplicon genes with the hypoxia gene expression signature in breast cancer (Spearman’s ρ: SQLE = 0.31, PYCRL = 0.25, TSTA3 = 0.25, CYC1 = 0.25, SLC39A4 = 0.24; P < 10−3), we tested whether these five genes are directly induced by hypoxia and/or, when expressed, confer survival advantage under hypoxia. This evidence concerns the gene PYCR3 and breast cancer.