We collected 53 pairs of primary human HCC samples with adjacent normal tissues, and determined protein changes (ACSS1, ACSS2 and FASN) and six histone acetylation marks (H3K9ac, H3K14ac, H3K18ac, H3K23ac, H3K27ac and H3K56ac) in all these samples (n=53) (Fig. 6a; Supplementary Fig. 6b). The gene discussed is ACSS2; the disease is hepatocellular carcinoma.