In contrast, RBPJ was nearly undetectable basally by immunostaining with the monoclonal antibody T6709 (ref. 14) (Fig. 1d), but was strikingly evident in response to a doxycyclin-inducible, transgenic NICD (iNICD)15 (Fig. 1c,f) and after TAC or myocardial infarction (MI) (Fig. 1e; Supplementary Fig. 1c–f) coinciding with elevated expression of Notch ligands (Supplementary Fig. 2a–d). The gene discussed is RBPJ; the disease is myocardial infarction.