Nuclear-localized and faint cytoplasmic RBPJ is readily detected in the adult heart, both in cardiomyocytes and non-myocytes, and its level does not change substantially following either chronic pressure overload by thoracic aortic constriction (TAC) or myocardial infarction (Fig. 1a,b; Supplementary Fig. 1). Here, RBPJ is linked to myocardial infarction.