TP53 and rectum adenocarcinoma: However, in rectum adenocarcinoma (READ), although R175 is associated with increases in TP53 RNA expressions similar to what is observed in BRCA, R248 and R273 missense mutations are not significantly associated with the TP53 mRNA or protein expression, comparing to samples with non-hotspot or no mutations in TP53 (Fig. 4a), implicating distinct functions of R248 and R273 in different disease contexts.