TP53 and cancer: Of the 21 hotspot mutations detected in TP53 (Fig. 6a), 2 were found to be prevalent in multiple cancer types (R248 in bladder urothelial carcinoma (BLCA), BRCA and OV, R273 in lower grade glioma (LGG), BRCA and OV), and 9 (G108, R158, R175, I195, R213, Y220, R249, R282, E285) in one tumor type, confirming the functional diversity of TP53 hotspot mutations in different cancer types (Fig. 4a).