Moreover, Ad-E2F1 significantly increased the expression of miR-15a, miR-16, and miR-29a in SaOS-2 cells, although MNNG/HOS cells showed increased expression of miR-15a and miR-16, but not miR-29a, after Ad-E2F1 infection (Fig. 5d). The gene discussed is E2F1; the disease is infection.