We observed that the samples with a MANF concentration of more than 13.5 ng/ml had been taken closer to the clinical diagnosis of T1D (3.3 ± 2.5 days; n = 23) compared to the samples with lower MANF levels (5.8 ± 3.6 days, n = 75; p < 0.001). Here, MANF is linked to type 1 diabetes mellitus.