1985). HOMA‐IR was significantly higher in Hfe+/− mice fed both CD (P = 0.038) and HCD (P= 0.039) using Dunn's multiple comparison test after a significant Kruskal–Wallis test (P = 0.0024) (Fig. 5C). Serum adiponectin has previously been shown to be regulated by iron in mice and is a known key determinant of insulin resistance (Gabrielsen et al. 2012). However, we did not find any significant effect of genotype on total serum adiponectin (Fig. 5D and E). This evidence concerns the gene ADIPOQ and Insulin resistance.