To validate the RNA-seq data, expression of some selected genes; c-MYB, ADM, ALDH1, BBC3 (PUMA), HDAC5, KLF4, LDHA, MDM2, SHH, SLC2A1 (GLUT1); relevant to PC pathobiology were examined by quantitative reverse transcription PCR (qPCR) using gene specific primer set. The gene discussed is BBC3; the disease is pachyonychia congenita.