To test if BRAF600E mediates ERK activation in colon cancer cells, we overexpressed the mTOR-insensitive and constitutively active form of S6K1 (HAS6K1 E389 delta CT) [28] and mTOR-sensitive WT S6K1 in 600E cells. The gene discussed is RPS6KB1; the disease is malignant colon neoplasm.