To verify such a hypothesis, a gene reporter assay was carried out in MTC cells transfected with a luciferase reporter plasmid bearing the wild-type form of the RET promoter (pGL3-RETAwt) or its mutated variant (pGL3-RETAmut) bearing G>A and G>T point mutations (Figure 3A). This evidence concerns the gene RET and medullary thyroid gland carcinoma.