TNF and type 2 diabetes mellitus: To explore the association of Src with β-cells mass and T2DM, especially its risk factors such as Glu-palm, LPS and TNF-α, MIN6 cells were subjected to different stimuli, such as treatment with 0.4 mM palmitate plus 16.7 mM glucose for 24 h, or 80 nM TNF-α for 6 h, or 10 μg/mL LPS for 24 h, according to our previous reports28, 30.