As shown in Fig. 5G, EGCG at a concentration of 100 μM combined with F2,6BP significantly attenuated EGCG-induced apoptosis in HCC-LM3 and HepG2 cells by inhibiting the activation of caspases 3, 8, and 9, confirming the involvement of PFK in EGCG-induced apoptosis. This evidence concerns the gene CASP3 and hepatocellular carcinoma.