To examine the mechanism underlying the effect of EGCG on inhibiting glycolysis in aerobic glycolytic HCC cells, the effects of EGCG on the expression of several key glycolytic enzymes, including GLUT1, GLUT2, GLUT3, GLUT4, HK2, PFK, PKM2, and LDH-A were assessed in HCC-LM3 and HepG2 cells using qRT-PCR. This evidence concerns the gene HK2 and hepatocellular carcinoma.