Figure 1a shows that expression of FFAR2 mRNA was reduced by more than twofold in 71% (10 out of 14) of the colon cancers than adjacent normal tissue. When data was pooled for these samples, FFAR2 expression was still significantly downregulated (by ~60%) in colon cancers than adjacent normal tissue (Figure 1b) (P<0.0001). Similarly, expression of FFAR3 mRNA was also downregulated in colon cancer samples (Supplementary Figure 1). Same set of colon cancer samples as used in current study also exhibits decreased expression of HCAR2 (GPR109A).33 This evidence concerns the gene HCAR2 and malignant colon neoplasm.