RASSF10 and hepatocellular carcinoma: To confirm whether the promoter methylation is involved in the silencing of RASSF10, five HCC cell lines (BEL7404, QGY7701, QGY7703, HepG2 and Hep3B) with silenced RASSF10 were treated with 5-Aza-DC for 72 h, which restored RASSF10 expression in all of them (Figure 1e).