PLAT and neoplasm: Excised Ric-8AFlox/Flox; Rosa-CreER+/−; Tg (GNAQQ209L) tumors from Figure 3a were cultured ex vivo in standard melanocyte culture medium lacking TPA to derive two independent GNAQQ209L melanoma cell lines, with which to determine the effect of Ric-8A deletion on secondary tumor progression.