The HFD used in our study contains a high percentage of saturated fatty acid, which is known to induce chronic inflammation in WAT, worsening insulin resistance.4 By contrast, n-3 PUFAs such as eicosapentaenoic acid (EPA) and docosahexaenoic acid have been reported to suppress chronic inflammation through various pathways involving Toll-like receptor 4, resolvin E1 and GRP120.5, 6, 7. Here, TLR4 is linked to Insulin resistance.